Marwa F. Abd Al-Munim, Nihad A.M. Al-Rashedi Received 10-31-2021, Accepted 10-26-2021, published 05-31-2022.
The stability of deoxyribonucleic acid (DNA) depends on several factors including temperature, humidity, and period conditions. Temperature plays an essential role in DNA degradation, the high relative humidity cause rapid decay of DNA. The aim of this study is to assess the effects of different conditions [Temperature (0°C and 55°C) and Humidity (58% and 12%)] on DNA methylation by bisulfite. The study has investigated 30 samples of blood that have been collected from healthy people. The samples were distributed for four groups each group has 15 samples were exposed at different conditions temperature (0°C, and 55°C) and humidity (58%, and 12%) in different time ( 0,1,2,4 and 8 days). The DNA methylation of TRIM 59 gene was stable in room temperature whereas in the 0°C and 55°C the results showed that a slight regression in TRIM59gene.
TRIM59, DNA methylation, Blood, Pyrosequencing, Temperature, Humidity
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