Muthanna Journal of Pure Sciences – MJPS
Volume 3 issue 1, 2016
Nouran Raad Jomah* Muhammed Oda Mallah**
College of Science-AL-Muthanna University, Iraq
Abstract
The aim of this study was to make comparison for prevalence of Cryptosporidium
parvum among children by traditional microscopic examination techniques
(Direct,Flotation,Sedimentation and Ziehl-Neelsen stain) as well as RT-PCR . This study
was carried out in AL-Muthanna province during the period from October 2013 to May
2014 in the Educational -AL- Hussein hospital in AL-Samawa, General AL-Rumaiytha
hospital, Feminine and Children’s hospital in AL-Samawa and AL-Rumaiytha health
centers. A total of 100 stool samples were taken from children aged 1 month to less than 10
years who suffering from acute or persistent diarrhea and it examined by Direct, Flotation
and Sedimentation methods and Ziehl-Neelsen stain and RT- PCR assay. The present study
recorded the prevalence percent of Cryptosporidium parvum that detected in 100 fecal
samples examined by direct , flotation and sedimentation methods were 30%, 36 and 40%
respectively. While in this study , the fecal samples of children were examined by Ziehl-
Neelsen, out of which 21 fecal samples had suspected Cryptosporidium parvum with
percent 21%. The results showed oocysts stained purple bodies against a dark blue back
ground, with a clear halo around the oocyst. Alsoin this study , the fecal samples of
children were examined by real time PCR out of which 18 fecal samples had found
Cryptosporidium parvum with percent 18%. The present study showed that high percentage
of positive cases was recorded in microscopical examination while 18% of positive cases
were detected by molecular assay.
Keyword: Cryptosporidium parvum, Children, Stool, RT-PCR, Iraq.