Muthanna Journal of Pure Sciences – MJPS
Volume 3 issue 1, 2016
Maan Abul-Milh
College of Dentistry, Al-Muthanna University, Al-Muthanna, Iraq
Abstract
Binding of Helicobacter pylori to glycolipids separated on thin-layer chromatogram and
incorporated into liposomes has been carried out. Wild type clinical isolates recognized
well galactosylceramide with non-hydroxylated fatty acid of ceramide [Galβ1Cer (non-
OH)] and weaker bound galactosylceramide with hydroxylated fatty acid[Galβ1Cer(OH)].
Rough and culture collection strainsdemonstrated contrarily different binding profile to
hydroxylated glucosylceramide [Glcβ1Cer (OH)] and hydroxylated galactosylceramide
[Galβ1Cer (OH)]in comparison with the wild type strains. All tested bacterial strainsfailed
to recognize glucosylceramide with non-hydroxylated fatty acid[Glcβ1Cer (non-OH)],
separated on TLC palte. Glucosylceramide [Glcβ1Cer (OH)] was bound likely well as
non-hydroxylated galactosylceramide [Galβ1Cer (non-OH)]by clinical isolates,
whereasbinding to these sugars was weak and variable when using rough and culture
collection strains. [Galβ1Cer (OH)] has been recognized by wild type strains in the
liposome assay, whereas[Glcβ1Cer (non-OH)] was detected weakly and variably by both
types of strains using liposome aggregation assay. Lactosylceramide (lacer),with
hydroxylated fatty acid of the ceramide [Galβ1-4Glcβ1Cer (OH)] was detected by bacteria
using TLC plate. Both clinical isolates andculture collection strainsstrongly recognized
lacCer with hydroxylated fatty acid of ceramide on thin-layer chromatogram. Lipids from
non-acid fraction of the same preparation were recognized by H. pylori characterized
bypositive inding to gangliotriaosylceramide, GgO3(GalNAcβ1-4Galβ1-4Glcβ1Cer) and
gangliotetraosylceramide, GgO4 (Galβ1-3GalNAcβ1-4Galβ1-4Glcβ1Cer).PE was the only
receptor active phospholipid detected byH. pylori. No binding was shown to globoseries
glycolipids-containing Galα1-4Gal.