Noor al-Huda Saber Sadiq1,*, Dhifaf Zeki Aziz1 1University of Kufa, Faculty of Science, Department of Pathological Analyses,Al-Najaf, Iraq.
*Corresponding author: nooralhadaa.alkhazrajy@student.uokufa.edu.iq
Received 20 May. 2025, Accepted 19 June. 2025, published 30 Dec. 2025.
AbstractKey wordsDOI
Hashimoto’s thyroiditis (HT) is an autoimmune disorder in which progressive thyroid failure is induced by Th1-type immunity, and in particular, interferon-gamma (IFN-γ). Immunological diagnosis of HT was made in the present study by comparing 50 patients with HT and 50 sex- and age-matched controls for serum IFN-γ, IL-18, anti-TPO, anti-Tg, hs-CRP, and TSH levels. Biomarkers were quantified using ELISA and Cobas e411 and statistically evaluated for group comparison, correlation, regression, and ROC curve. All biomarkers were significantly higher in HT patients (p < 0.001). IFN-γ was strongly correlated with IL-18, anti-TPO, and anti-Tg (r > 0.7) and had high diagnostic accuracy (AUC = 0.92; cutoff = 59.96 pg/mL). These observations implicate IFN-γ as a key mediator of HT pathogenesis and suggest its utility in diagnosis and in the design of future immunomodulatory treatment strategies.
Hashimoto’s thyroiditis, interferon-gamma, interleukin-18, hs-CRP, cytokine profiling. acknowledgments.
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